Fig 7.

Virus replication studies. (A) PBMC isolated from cynomolgus macaques were infected with equal amounts of 293T-produced SIVmac239 (wt) and SIVmac239-m4 (m4) virus (5 ng CA-p27). Replication was monitored by measuring the RT level in the culture supernatant. The results shown are representative of 2 independent experiments, and similar results were obtained with different amounts of virus input. (B) Virus competition experiments were conducted in 174×CEM cells. Pairwise competitions were started by transfecting an equimolar mixture of wt and mutant viral DNA constructs as previously described (27, 55, 56). The proviral DNA was analyzed by sequencing at several time points. The original input DNA (day 0) was included as a control, in which the double A/U signal (arrow) demonstrates the presence of a wt-m4 mixture.