Table 3.
Host factors from a ribosomal salt wash of rabbit reticulocyte lysates that interacted with the 5′-extremity of the murine norovirus genomea
| Band no. | Protein ID | Accession no. | Mass (kDa) | SC (%) | No. of unique peptides |
Identification probability (%) |
||
|---|---|---|---|---|---|---|---|---|
| 5′G | NR | 5′G | NR | |||||
| 1 | HNRNPK | B2M1R6 | 49 | 23 | 7 | 0 | 100 | 0 |
| YBX1 | B5ABI3 | 36 | 14 | 2 | 2 | 100 | 99 | |
| CCT2 | P80314 | 57 | 7 | 2 | 0 | 75 | 0 | |
| TCP-1-ζ | O77622 | 58 | 6 | 3 | 0 | 100 | 0 | |
| CCT4 | A8K3C3 | 58 | 5 | 2 | 0 | 100 | 0 | |
| 2 | CSNK2A1 | Q61177 | 45 | 37 | 13 | 1 | 100 | 89 |
| 3 | PCBP1 | P60335 | 37 | 37 | 9 | 5 | 100 | 100 |
| CSNK2A1 | Q61177 | 45 | 34 | 9 | 0 | 100 | 0 | |
| PCBP2 | B2M1R7 | 38 | 21 | 2 | 0 | 100 | 0 | |
| YBX1 | B5ABI3 | 36 | 11 | 2 | 4 | 100 | 100 | |
| 4 | CSNK2B | Q5SRQ6 | 25 | 42 | 8 | 0 | 100 | 0 |
Protein ID lists the UniProtKB accession number for each of the proteins identified in Fig. 2B. SC refers to the percent sequence coverage for the protein purified and identified using the 5′ extremity of the MNV genomic RNA (5′G). NR refers to the data acquired when we used a nonrelated RNA, as described in the text. The identification probability was calculated using the ProteinProphet algorithm. Samples were arranged per gel slice excised from the gel presented in Fig. 2B and then ranked based on the number of unique peptides identified.