Table I.
Chlorophyll DBC Dose-Dose Matrix Experimental Design
| Group | tanks | 3 days pre-fed | 4 weeks co-fed | 3 days post-fed |
|---|---|---|---|---|
| 1 | 2 | OTD | OTD | OTD |
| 2 | 2 | Chl 4000 ppm | Chl 4000 ppm | Chl 4000 ppm |
| 3 | 3 | OTD | DBC 14 ppm | OTD |
| 4 | 6 | OTD | DBC 28 ppm | OTD |
| 5 | 3 | OTD | DBC 56 ppm | OTD |
| 6 | 6 | OTD | DBC 112 ppm | OTD |
| 7 | 3 | OTD | DBC 224 ppm | OTD |
| 8 | 6 | Chi 500 ppm | Chi 500 ppm & DBC 14 ppm | Chi 500 ppm |
| 9 | 6 | Chi 500 ppm | Chi 500 ppm & DBC 28 ppm | Chi 500 ppm |
| 10 | 3 | Chi 500 ppm | Chi 500 ppm & DBC 56 ppm | rChl 500 ppm |
| 11 | 3 | Chi 500 ppm | Chi 500 ppm & DBC 112 ppm | Chi 500 ppm |
| 12 | 3 | Chi 500 ppm | Chi 500 ppm & DBC 224 ppm | Chi 500 ppm |
| 13 | 6 | Chl 1000 ppm | Chi 1000 ppm & DBC 28 ppm | Chl 1000 ppm |
| 14 | 3 | Chl 1000 ppm | Chi 1000 ppm & DBC 56 ppm | Chl 1000 ppm |
| 15 | 3 | Chl 1000 ppm | Chi 1000 ppm & DBC 112 ppm | Chl 1000 ppm |
| 16 | 3 | Chl 1000 ppm | Chi 1000 ppm & DBC 224 ppm | Chl 1000 ppm |
| 17 | 6 | Chl 2000 ppm | Chl 2000 ppm & DBC 28 ppm | Chl 2000 ppm |
| 18 | 3 | Chl 2000 ppm | Chl 2000 ppm & DBC 56 ppm | Chl 2000 ppm |
| 19 | 3 | Chl 2000 ppm | Chl 2000 ppm & DBC 112 ppm | Chl 2000 ppm |
| 20 | 3 | Chl 2000 ppm | Chl 2000 ppm & DBC 224 ppm | Chl 2000 ppm |
| 21 | 6 | Chl 4000 ppm | Chl 4000 ppm & DBC 56 ppm | Chl 4000 ppm |
| 22 | 3 | Chl 4000 ppm | Chl 4000 ppm & DBC 112 ppm | Chl 4000 ppm |
| 23 | 3 | Chl 4000 ppm | Chl 4000 ppm & DBC 224 ppm | Chl 4000 ppm |
| 24 | 3 | Chl 4000 ppm | Chl 4000 ppm & DBC 448 ppm | Chl 4000 ppm |
| 25 | 6 | CHL 2000 ppm | CHL 2000 ppm & DBC 112 ppm | CHL 2000 ppm |
| 26 | 3 | CCC Chl 2000 ppm | CCC Chl 2000 ppm & DBC 112 ppm | CCC Chl 2000 ppm |