Table II.
Tumor Incidence, liver somatic index, and tumor type among animals fed varying doses of DBC and Chlorophyll
| [DBC] (ppm) | [Chla] (ppm) | N | Tumor Incidencea |
Mean Body Weight (g)a | Liver Somatic Indexa,b | # Livers with tumors Examined | % of Liver Tumors Examinedc |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Liver | Stomach | HCC | HCA | MC | MA | CCC | CCA | ||||||
| 0 | 0 | 186 | _ | _ | 122.5 (8.1) | 0.73 (0.01) | _ | _ | _ | _ | _ | _ | _ |
| 0 | 4000 | 194 | _ | 1.6 (0.6) | 117.1 (8.7) | 0.70 (0.02) | _ | _ | _ | _ | _ | _ | _ |
| 14 | 0 | 297 | 2.7 (0.3) | 3.0 (0.6) | 116.5 (3.3) | 0.71 (0.01) | 8 | 62.5 | 25.0 | 0 | 12.5 | 0 | 0 |
| 28 | 0 | 575 | 10.8 (1.8) | 0.9 (0.3) | 117.9 (2.9) | 0.74 (0.02) | 62 | 69.4 | 16.1 | 11.3 | 1.6 | 0 | 1.6 |
| 56 | 0 | 284 | 29.9 (1.8) | 4.2 (1.1) | 118.9 (2.6) | 0.72 (0.04) | 85 | 45.9 | 37.6 | 10.6 | 3.5 | 0 | 2.4 |
| 112 | 0 | 574 | 56.2 (2.7) | 23.5 (1.0) | 115.0 (3.4) | 0.72 (0.01) | 322 | 57.5 | 23.6 | 17.7 | 0.9 | 0.3 | 0 |
| 224 | 0 | 289 | 37.1 (2.6) | 46.5 (3.1) | 114.4 (2.3) | 0.73 (0.02) | 107 | 58.9 | 34.6 | 4.7 | 1.9 | 0 | 0 |
| 14 | 500 | 568 | 0.9 (0.4) | 1.3 (0.8) | 118.8 (3.6) | 0.72 (0.01) | 5 | 20.0 | 80.0 | 0 | 0 | 0 | 0 |
| 28 | 500 | 560 | 4.1 (0.9) | 0.7 (0.2) | 115.0 (1.7) | 0.72 (0.02) | 23 | 87.0 | 8.7 | 4.3 | 0 | 0 | 0 |
| 56 | 500 | 282 | 15.2 (4.0) | 2.8 (1.3) | 117.2 (1.9) | 0.71 (0.01) | 43 | 65.1 | 18.6 | 16.3 | 0 | 0 | 0 |
| 112 | 500 | 286 | 45.2 (1.9) | 11.8 (2.2) | 115.9 (3.4) | 0.71 (0.01) | 129 | 42.6 | 39.5 | 16.3 | 0.8 | 0 | 0.8 |
| 224 | 500 | 284 | 42.1 (2.2) | 20.2 (1.6) | 114.3 (5.1) | 0.74 (0.02) | 119 | 57.1 | 32.8 | 9.2 | 0 | 0.8 | 0 |
| 28 | 1000 | 566 | 3.0 (1.0) | 0.8 (0.5) | 117.9 (2.7) | 0.72 (0.02) | 17 | 41.2 | 41.2 | 11.8 | 5.9 | 0 | 0 |
| 56 | 1000 | 296 | 12.8 (3.2) | 2.1 (0.6) | 115.1 (5.8) | 0.72 (0.03) | 37 | 54.1 | 29.7 | 13.5 | 0 | 2.7 | 0 |
| 112 | 1000 | 250* | 39.1 (4.5) | 12.2 (2.2) | 116.8 (5.5) | 0.67 (0.05) | 101 | 45.5 | 38.6 | 12.9 | 1.0 | 2.0 | 0 |
| 224 | 1000 | 274 | 39.1 (3.1) | 40.8 (2.1) | 119.0 (3.0) | 0.71 (0.04) | 108 | 44.4 | 34.3 | 18.5 | 2.8 | 0 | 0 |
| 28 | 2000 | 584 | 2.9 (0.8) | 2.1 (0.5) | 116.9 (1.9) | 0.71 (0.01) | 17 | 11.8 | 58.8 | 29.4 | 0 | 0 | 0 |
| 56 | 2000 | 217* | 9.0 (0.7) | 3.2 (1.2) | 118.1 (1.8) | 0.64 (0.02) | 19 | 47.4 | 36.8 | 10.5 | 0 | 0 | 5.3 |
| 112 | 2000 | 279 | 30.8 (3.1) | 14.3 (1.0) | 120.2 (1.2) | 0.69 (0.01) | 86 | 57.0 | 33.7 | 8.1 | 1.2 | 0 | 0 |
| 224 | 2000 | 292 | 42.1 (3.4) | 36.7 (2.3) | 117.5 (3.9) | 0.69 (0.01) | 122* | 56.6 | 28.5 | 13.1 | 0.8 | 0.8 | 0 |
| 56 | 4000 | 580 | 4.2 (1.4) | 1.2 (0.6) | 114.8 (1.8) | 0.72 (0.01) | 25 | 32.0 | 40.0 | 12.0 | 16.0 | 0 | 0 |
| 112 | 4000 | 295 | 14.5 (2.3) | 8.1 (2.0) | 114.7 (2.6) | 0.70 (0.03) | 44 | 40.9 | 38.6 | 15.9 | 2.3 | 0 | 2.3 |
| 224 | 4000 | 287 | 46.3 (3.5) | 24.4 (2.4) | 115.8 (2.6) | 0.70 (0.02) | 133 | 51.9 | 30.8 | 15.8 | 1.5 | 0 | 0 |
| 448 | 4000 | 300 | 29.6 (3.0) | 54.3 (4.2) | 111.3 (1.1) | 0.71 (0.01) | 89 | 50.6 | 38.2 | 11.2 | 0 | 0 | 0 |
| 112 | CCC | 303 | 14.2 (2.5) | 6.6 (1.2) | 113.6 (3.2) | 0.69 (0.01) | 43 | 53.5 | 18.6 | 18.6 | 9.3 | 0 | 0 |
| 112 | CHL | 568 | 12.3 (2.3) | 3.4 (0.7) | 113.8 (2.6) | 0.70 (0.01) | 69 | 55.1 | 33.3 | 10.1 | 0 | 0 | 1.4 |
a
Mean (SE);
b
Somatic index is defined as mean liver weight (g)/ mean body weight (g).
c
HCC, hepatocellular carcinoma; HCA, hepatocellular adenoma; MC, mixed hepatocellular/ cholangiocellular carcinoma; MA, mixed hepatocellular/cholangiocellular carcinoma; CCC, cholangiocellular carcinoma; CCA, cholangiocellular adenoma.
d
'_' indicates that no tumors were observed.
*
Loss of trout due to error in husbandry.
°
122 of the 123 livers with tumors were classified as to tumor type.