Table III.
Genes upregulated by dietary exposure to DBC compared to chemo-intervention
| Mean fold change in gene expressiona a | ||||
|---|---|---|---|---|
| Gene | Annotation (DFCI accession / GenBank accession) | DBC b | DBC+Chl c | Chl d |
| Extracellular, environmental response | ||||
| ARNT | TC142567 / U73841.1 | 1.76 | - | - |
| Growth factor | ||||
| EGFR | FM865854 | 1.43 | - | - |
| Immune response | ||||
| IFITM3 | TC146120 / AJ320157 | 1.29 | - | - |
| PSME1 | BT074326 | 1.00 | - | - |
| PSME2 | BT074299 | 1.00 | - | - |
| cf H | TC162759 / AJ627206 | 1.12 | - | - |
| CD63 | TC137037 / AY593998 | 1.00 | - | - |
| MHC I | TC132378 / AB012064 | 1.24 | - | - |
| HAMP | TC164847 / AF281354 | 1.02 | ||
| Cell proliferation/apoptosis | ||||
| P53 | TC132531 / NM_001124692 | 2.28 | - | - |
| PCNA | NM_131404 | 1.43 | - | - |
| MAPK8 | CX028516 | 1.34 | - | - |
| Metabolism | ||||
| CYP1A1 | TC158463 / U62796 | 2.79 | - | - |
| SULT1A2 | TC135750 / BX867991 | 1.02 | - | - |
| GAPD | TC137453 / NM_001124246 | 1.00 | - | - |
| DLAT | TC132429 / J04051 M29099 | 1.16 | - | - |
| SOD1 | NP543739 / AF469663 | 1.39 | - | - |
| Transport proteins | ||||
| FXYD | TC165305 / BT074290 | 1.81 | - | - |
| Elongation factors | ||||
| EF-1 alpha | TC166688 / AF498320 | 1.31 | - | - |
| Extracellular matrix | ||||
| C-type lectin | NM_001160495 | 1.00 | ||
| MMP9 | TC132759 / AJ320533 | 1.12 | - | - |
| Bone and cartilage maintenance | ||||
| Cathepsin K | TC8255 / U61499 | 1.45 | - | - |
| Function in trout unknown | ||||
| Cathepsin S | TC8256 / AY622858 | 1.17 | - | - |
| General cell signaling | ||||
| RhoG | TC169097 / BE859102 | 1.03 | - | - |
a
Mean fold changes in gene expression were log2 transformed. Only probes that differed by an average of at least twofold between treatment and control groups and were significant (P<0.05) are reported.
b
224 ppm DBC was administered via the diet for 20 days and liver samples for RNA were collected at the end of the 20 day exposure period.
c
224 ppm DBC + 2000 ppm Chl were co-administered via the diet for 20 days and liver samples for RNA were collected at the end of the 20 day exposure period.
d
2000 ppm Chl were co-administered via the diet for 20 days and liver samples for RNA were collected at the end of the 20 day exposure period.