Fig 1.

Overexpression of llmg2447 reduces susceptibility to Lcn972. (A) Domain architecture of Llmg2447 (368 aa) and its truncated version Llmg2447_1-115, which are encoded by pBL51 and pBL51Cla, respectively. TMR, transmembrane region as predicted by TMHMM Server v2.0 and SOSUI (see the text). C-terminal residues that are encoded by vector sequences are shown in the shaded box. WT, wild type. (B) Fold increase in resistance to Lcn972, relative to that under noninducing conditions (0 ng/ml nisin), of L. lactis carrying the empty vector (pNZ8020) or llmg2447-expressing vector pBL51 or pBL51Cla after induction with several concentrations of nisin (inset). Resistance of the Lcn972-resistant mutants L. lactis D1 and D1-20 is given relative to that of their parent, L. lactis MG1614. The increase in L. lactis D1 resistance was 400-fold and is indicated by the broken column. Results of a representative experiment are shown.