Table 1.
Strain or plasmid | Descriptiona | Reference |
---|---|---|
L. lactis | ||
MG1363 | Plasmid-free derivative of L. lactis NCDO712 | 16 |
MG1614 | Strr Rifr derivative of L. lactis MG1363, Lcn972s | 16 |
D1 | MG1614, highly Lcn972-resistant mutant | 34 |
D1-20 | MG1614, low Lcn972 resistance mutant | 34 |
NZ9000 | MG1363, pepN::nisRK; host for nisin-inducible gene expression | 12 |
IL1403 | Plasmid free, complete ECF sigX gene | 2 |
E. coli DH10B | Plasmid free, cloning host | Invitrogen |
Plasmids | ||
pCR2.1 | Cloning of PCR products, Apr | Invitrogen |
pNZ8020 | Nisin-inducible promoter PnisA, Cmr | 12 |
pPTPL | lacZ reporter plasmid, Tetr | 8 |
pBL51 | pNZ8020::llmg2447 | This work |
pBL51Cla | pBL51ΔClaI site, frameshift in llmg2447 | This work |
pBL56 | pCR2.1::wild-type llmg2447 promoter (P2447) | This work |
pBL57 | pCR2.1::hybrid IS981-llmg2447 promoter (PIS981::2447) | This work |
pBL58 | pPTPL::wild-type llmg2447 promoter (P2447) | This work |
pBL59 | pPTPL::hybrid IS981-llmg2447 promoter (PIS981::2447) | This work |
Str, streptomycin; Rif, rifampin; Em, erythromycin; Ap, ampicillin; Tet, tetracycline; superscript r, resistant; superscript s, susceptible.