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. 2012 Nov;56(11):5520–5527. doi: 10.1128/AAC.01206-12

Table 2.

Primers used in this work

Primer 5′–3′ sequence (underlined restriction site) Position
X1 TTTTGTCTGAATTCCAAAACTCTT (EcoRI) 5′ llmg2447 promoter
X2 CCGGATCCATTTCCTCCCCTCTC (BamHI) 3′ llmg2447 promoter
X3 TGTTCGATACATAAAAGCCCG 3′ llmg2447 promoter
X4 ATCTGCAGTGGCTCCTCTAATC (PstI) 5′ llmg2447 gene
X5 GCAAAGAATTCAATGACCGCCT (EcoRI) 3′ llmg2447 gene
X6 ACGAATTCATCAAAGTTTAGGGTATC (EcoRI) 5′ IS981::2447 promoter