Table 2.
Primer | 5′–3′ sequence (underlined restriction site) | Position |
---|---|---|
X1 | TTTTGTCTGAATTCCAAAACTCTT (EcoRI) | 5′ llmg2447 promoter |
X2 | CCGGATCCATTTCCTCCCCTCTC (BamHI) | 3′ llmg2447 promoter |
X3 | TGTTCGATACATAAAAGCCCG | 3′ llmg2447 promoter |
X4 | ATCTGCAGTGGCTCCTCTAATC (PstI) | 5′ llmg2447 gene |
X5 | GCAAAGAATTCAATGACCGCCT (EcoRI) | 3′ llmg2447 gene |
X6 | ACGAATTCATCAAAGTTTAGGGTATC (EcoRI) | 5′ IS981::2447 promoter |