Fig 5.

Growth complementation of auxotrophism by isogenic and nonisogenic P. aeruginosa prototrophic isolates (over a 6-mm sterile absorbent filter disc labeled “Feeder”) inoculated onto M9 agar medium. (A to F) Examples of negative (A and B) and positive (C, D, E, and F) growth complementation of an auxotrophic strain by various prototrophic P. aeruginosa strains. (E and F) Examples of close-up views (stereoscopy at ×30 magnification) of growth complementation patterns of an auxotrophic strain by prototrophic strains that were either isogenic (E) or nonisogenic (F). (G) An example of contact inhibition of wild-type PA14 growth by the auxotrophic PA-C4. (H) An example of nonhomogeneous growth of PA-C6 on M9 agar medium (×60 magnification).