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Determination of the Ki of AFN-1252 for S. aureus FabI. Crotonyl-ACP (substrate) concentrations [S]/reaction velocity (V) were plotted against AFN-1252 (inhibitor) concentrations at three different substrate concentrations, i.e., 6.1 μM (▲), 25 μM (■), and 50 μM (⧫).
