Fig 3.
Membrane potential in isolated S. cerevisiae mitochondria after addition of T-2307 or pentamidine. The mitochondrial membrane potential depolarization achieved by T-2307 (A) or pentamidine (B) was monitored by the increase of diS-C3-(5) fluorescence intensity. Wavelengths used for diS-C3-(5) were as follows: excitation (λEX) = 620 nm, and emission (λEM) = 670 nm. Fully depolarized mitochondria were obtained by incubation with 10 μM CCCP. The values are the averages for three independent experiments. A.U., arbitrary units.