Fig 4.
Membrane potential in isolated rat liver mitochondria after addition of T-2307 or pentamidine. The mitochondrial membrane potential depolarization achieved by T-2307 (A) or pentamidine (B) was monitored by the increase of diS-C3-(5) fluorescence intensity. Wavelengths used were as follows: λEXC = 620 nm, and λEM = 670 nm [for diS-C3-(5)]. Fully depolarized mitochondria were obtained by incubation with 10 μM CCCP. The values are the average for three independent experiments. A.U., arbitrary units.