Table 1.
Oligonucleotide used in this study
| Genea | Gene namea | Direction | Primer sequence (5′–3′)b |
|---|---|---|---|
| SA0905 | atlA | Forward | GCTAATCAATCAGCGACTAC |
| Reverse | CTAATACGACTCACTATAGGGAGACAAATGCATGTACGAATGCG | ||
| SERP0636 | atlE | Forward | GGGACGTCCTGAAGGTATCG |
| Reverse | CTAATACGACTCACTATAGGGAGAGATGTTGTGCCCCAAGGTGC | ||
| SA2459 | icaA | Forward | GCCAACGCACTCAATCAAGG |
| Reverse | CTAATACGACTCACTATAGGGAGACTTCCAAAGACCTCCCAATG | ||
| SERP2293 | icaA | Forward | GAGGGAATCAAACAAGCATC |
| Reverse | CTAATACGACTCACTATAGGGAGACCATCGAACCCTTTGTTTCC |
a
Based on KEGG classification (www.genome.jp/kegg/).
b
The underlined sequence portions at the 5′ ends represent the recognition sequence for the T7 RNA polymerase.