Fig 2.

Ability of AL18 to inhibit influenza A polymerase activity in minireplicon assays. HEK 293T cells were cotransfected with plasmids encoding PB1, PB2, PA, and NP along with a plasmid carrying the firefly luciferase reporter gene flanked by the noncoding sequences of influenza A segment 8 and treated with the indicated compounds. The transfection mixtures also contained a plasmid constitutively expressing Renilla luciferase which served to normalize variations in transfection efficiency. Luciferase activity was quantified at 24 h posttransfection. The data are the means ± SDs of three independent experiments plotted relative to the activity seen in the presence of the compound vehicle (dimethyl sulfoxide [DMSO]). Omission of PB2 served as a negative control.