Table 1.
B. subtilis strains, plasmids, and oligonucleotides used in this study
| Strain, plasmid, or oligonucleotide | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| PDC134 | W168 trpC2 Ω spoVD::Cm Pxyl-murE Ω amyE::xylR | 19 |
| PDC204 | W168 trpC2 Ω spoVD::Erm Pxyl-murE Ω | 19 |
| RM29 | W168 trpC2 Ω spoVD::Cm Pxyl-murE Ω amyE::xylR sacB::Spec | R. Mercier |
| TMB1075 | PDC204 Ω spoVD::Erm Pxyl-murE Ω amyE::pTM1 | This study |
| TMB1077 | PDC204 Ω spoVD::Erm Pxyl-murE Ω amyE::pER603 | This study |
| TMB1080 | PDC204 Ω spoVD::Erm Pxyl-murE Ω amyE::pTM1, sacB::Spec | This study |
| TMB1082 | PDC204 Ω spoVD::Erm Pxyl-murE Ω amyE::pER603, sacB::Spec | This study |
| Plasmids | ||
| pAC6 | Bla amyE′ lacZ Cat ′amyE (integrative promoter probe vector) | 69 |
| pTM1 | pAC6 PliaI-lacZ | 30 |
| pER603 | pAC6 PbceA-lacZ | 55 |
| Oligonucleotides | ||
| liaI-fwd(RT) | GGTATCGGAGCCATTATGCTC | This study |
| liaI-rev(RT) | CCCATTCGTCATCAAAGTGAG | This study |
| bceA-fwd(RT) | CGTCAGTATTATGGGTGCTTC | This study |
| bceA-rev(RT) | GAATGGTTCCGTGACTGACCTG | This study |
a
Resistance cassettes: Bla, ampicillin; Cat, chloramphenicol; Spec, spectinomycin; Erm, erythromycin.