Table 1.
Isolate | Time point of isolation from blood | Daptomycin MIC (μg/ml) | Mean cytochrome c bindingc ± SD | Mean polarization indexd ± SD | Mean cell wall thicknesse ± SD | % of cells with septum formation | Mean % survival in presence of LL37 ± SD |
---|---|---|---|---|---|---|---|
5938 | Initial | 192 | 0.378 ± 0.001 | 0.232 ± 0.011 | 33.6 ± 4.6 | 57.8a | 75.3 ± 4.6a |
8019 | +4 mo | 4 | 0.363 ± 0.009 | 0.232 ± 0.018 | 33.6 ± 4.5 | 46.6 | 50.3 ± 6.0 |
5994 | +8 mo | 32 | 0.413 ± 0.003 | 0.224 ± 0.008 | 37.3 ± 5.5a | 50.4 | 108 ± 9.4b |
Significantly different (P < 0.05) from strain 8019.
Significantly different (P < 0.05) from both strains 8019 and 5938.
Cytochrome c binding results are expressed as units of optical density at 530 nm of unbound cytochrome c in supernatant.
The polarization index is expressed as degree of fluorescence polarization (4).
Cell wall thickness is expressed in nm.