Fig 5.

(a to d) Immunostaining for VM in the liver of VM-administered rats. ICC was carried out using a dilution (1:60) of the culture supernatant of the AVM-113 MAb following digestion of sections with protease at 0.004% at 30°C for 1 h. (a) Lower magnification. Moderate immunostaining occurred in the sinusoidal capillaries and in Kupffer cells. (b) Higher magnification. Note that there is no staining in the hepatocytes. (c) Immunostaining in the macrophages around blood vessels in the connective tissues. (d) Staining was completely abolished by absorption of the MAb with VM-GMBS-BSA (30 μg/ml). Arrows, sinusoidal capillaries; arrowheads, Kupffer cells. Bars in panels a, c, and d = 20 μm. Bar in panel b = 10 μm.