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N. fowleri viability determination by the trypan blue exclusion method. Trophozoites were incubated with different concentrations of corifungin for 72 h. (A) Amebae with fresh medium only. (B) Amebae with 3.12 μM corifungin. (C) Amebae with 6.25 μM corifungin. (D) Amebae with 12.5 μM corifungin. (E) Amebae with 18.75 μM corifungin. (F) Amebae with 25 μM corifungin. Cells stained blue were considered nonviable. Magnification, ×40.
