Fig 3.

Pharmacokinetics of D3112 in mice and flies. (A, B, and C) Pharmacokinetics of D3112 in mice. Phage samples (2 × 10⁷ PFU in PBS) of D3112 were administered intramuscularly (i.m., open symbols) or intraperitoneally (i.p., solid symbols) into uninfected mice. Groups of mice (n = 3) were sacrificed at 0.5, 12, 24, 36, and 48 h postinfection, blood (A), lung (B), and liver (C) were extracted, and their homogenates were used to determine the PFU per unit volume (ml) as described in Materials and Methods. The PFU/ml are shown on a log scale. (D) Pharmacokinetics of D3112 in flies. Phage samples (5 × 10⁹ PFU in PBS) of D3112 were overlaid on the surface of the fly media for 12 h before flies were transferred to new media without phage. Groups of flies (n = 5) were collected at 0.5, 12, 24, 36, and 48 h, and their homogenates were removed to determine the PFU per fly, which are shown on a log scale.