(A) PFGE (XbaI restricted) of Kp154 (lane 2) and Kp141 (lane 3). Lanes 1 and 10, lambda ladder (New England BioLabs, Ipswich, MA). (B and C) PFGE of plasmids after S1 treatment and a Southern blot after blaKPC labeling. Lanes 4 and 11, Ec136; lanes 5 and 12, Ec136-TC; lanes 6 and 13, Kp141; lanes 7 and 14, Kp141-TC; lanes 8 and 15, Kp154; lanes 9 and 16, Kp154.