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SDS-PAGE showing total proteins from freeze-thaw supernatants. Lane 1, uninduced pZA24-KPC-9 (due to its leaky expression, a faint band is seen at 28.5 kDa); lane 2, Precision Plus Protein standards (Bio-Rad, Hercules, CA); lane 3, induced pZA24-KPC-3; lane 4, induced pZA24-KPC-9; lane 5, induced pZ-Bgl (an identical plasmid expressing a higher-molecular-mass protein; no protein is seen at the size of KPC).
