Fig 3.

Effect of sub-MIC nitroxoline on biofilm morphology. Biofilms were grown under static conditions on tissue culture flasks for 18 h in the absence (A, C, E, and G) or presence of 8 μg/ml nitroxoline (B, D, F, and H). (A and B) CLSM images at 100-fold original magnification of acridine orange-stained biofilm. Horizontal optical sections from the midpoint of the biofilms are shown on the left and vertical optical sections on the right. SEM images at 500-fold (C and D), 2,000-fold (E and F) and 10,000-fold (G and H) original magnifications.