Fig 8.
Effect of physiological concentrations of nitroxoline, colistin, and ciprofloxacin on preexisting biofilm. Biofilms of strains PAO1 (A to D) and BK6695-10 (E to H) were grown in x-well tissue culture coverglass chambers (Sarstedt) for 18 h, treated with the compounds indicated for 6 h, and dyed by LIVE/DEAD BacLight bacterial viability kit (Invitrogen). CLSM was performed as z-stacks of 70 μm, with 28 layers each. Images are shown as superposition in the x-z plane (left) and as sections of approximately 30 m2 (basal layers; 6 μm above the glass surface) (right). Living cells are colored green (SYTO 9), and dead cells are colored red (propidium iodide).