Fig 1.

Impact of intrinsic aminoglycoside resistance gene knockouts on gentamicin-promoted outer membrane permeability. Membrane permeability, as assessed by NPN fluorescence, was measured over time following exposure of wild-type (WT; strain K767) and mutant P. aeruginosa strains to 5 μg/ml (A) and 20 μg/ml (B) of gentamicin (added at the time indicated by the arrow). The strains listed at the right (top to bottom) correspond to the traces at the left (top to bottom, respectively) at the final time points. Data for wild-type strain K767 not treated with gentamicin (no GEN) are also shown. The data are representative of at least 2 independent experiments for each strain.