Table 4.
Impact of loss of multiple intrinsic aminoglycoside resistance genes on aminoglycoside susceptibility in P. aeruginosa
| Strain | Relevant property | MIC (μg/ml)a |
||||||
|---|---|---|---|---|---|---|---|---|
| GEN | PAR | SPC | KAN | TOB | AMI | STR | ||
| K767 | Wild type | 2 | 256 | 512 | 64 | 1 | 2 | 32 |
| K3160 | PstB− | 0.5 (4) | 32 (8) | 256 (2) | 16 (4) | 0.5 (2) | 0.5 (4) | 8 (4) |
| K3179 | PstB−, LptA− | 0.25 (8) | 16 (16) | 256 (2) | 16 (4) | 0.25 (4) | 0.25 (8) | 2 (16) |
| K3180 | PstB−, FaoA− | 0.25 (8) | 16 (16) | 256 (2) | 8 (8) | 0.125 (8) | 0.25 (8) | 2 (16) |
| K3181 | PstB−, PA0392− | 0.25 (8) | 8 (32) | 128 (4) | 8 (8) | 0.125 (8) | 0.25 (8) | 2 (16) |
| K3182 | PstB−, PA2798− | 0.125 (16) | 4 (64) | 64 (8) | 4 (16) | 0.0625 (16) | 0.125 (16) | 2 (16) |
| K3161 | FaoA− | 0.5 (4) | 16 (16) | 512 (1) | 16 (4) | 0.5 (2) | 1 (2) | 8 (4) |
| K3165 | LptA− | 0.5 (4) | 64 (4) | 512 (1) | 16 (4) | 0.5 (2) | 0.5 (4) | 8 (4) |
| K3164 | PA0392− | 0.5 (4) | 32 (8) | 256 (2) | 16 (4) | 0.5 (2) | 1 (2) | 8 (4) |
| K3185 | FaoA−, LptA− | 0.125 (16) | 8 (32) | 256 (2) | 8 (8) | 0.0625 (16) | 0.25 (8) | 2 (16) |
| K3184 | FaoA−, PA0392− | 0.25 (8) | 16 (16) | 128 (4) | 8 (8) | 0.125 (8) | 0.25 (8) | 4 (8) |
| K3183 | LptA−, PA0392− | 0.25 (8) | 16 (16) | 256 (2) | 16 (4) | 0.125 (8) | 0.25 (8) | 4 (8) |
a
Fold changes in MIC for the mutants relative to that for wild type are indicated in parentheses. GEN, gentamicin; PAR, paromomycin; SPC, spectinomycin; KAN, kanamycin; TOB, tobramycin; AMI, amikacin; STR, streptomycin.