Table 3.
Stability of the V-073 resistance phenotype upon cell culture passagea
| Virus | Amino acid change | EC50 (μM) |
Passage 10 (% resistant)b | |
|---|---|---|---|---|
| Passage 1 | Passage 10 | |||
| VDPV-1 10235 (parent) | 0.018 | NAc | NA | |
| 10235.1 | VP1 I194F | >10 | >10 | 82 |
| 10235.3 | VP3 A24V | 1.4 | 0.95 | 69 |
| VDPV-2 10230 (parent) | 0.036 | NA | NA | |
| 10230.4 | VP1 I194 M | 1.5 | 3.0 | 43 |
| 10230.8 | VP3 A24V | >10 | 0.31 | 93 |
| VDPV-3 10805 (parent) | 0.029 | NA | NA | |
| 10805.1 | VP1 I192F | >10 | >10 | 97 |
| 10805.5 | VP3 A24V | 1.1 | 1.0 | 37 |
a
Passage was performed in the absence of drugselection.
b
Resistance was determined at 10× the parental EC50.
c
NA, not applicable.