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2-DG inhibits angiogenic gene expression. mECK36 (A) and iSLKrK (B) cells were lytically induced by butyrate (3 mM) (A) or by doxycycline (1 μg/ml) (B) in the presence (black bars) or absence (white bars) of 2-DG (3 mM) for 24 h. qRT-PCR was performed to detect levels of gene expression associated with angiogenesis (duplicates ± SD). β-Actin and GAPDH were used as loading controls.
