Alleviating effect of each dose of fractions on (a) dermatitis score, (b) Ear thickness and (c) scratching behavior. At the day 7, 14, 21, and 28 of treatment, clinical score and thickness of DNCB treated right ear of NC/Nga mice were measured. (a) Clinical skin score was evaluated as sum of the scores in five clinical symptoms. (b) Ear thickness was measured by dial thickness gauge. Both clinical skin score and ear thickness are expressed as mean ± SEM of five mice per group. Both clinical skin score and ear thickness are expressed as mean ± SEM of five mice per group. ###: P < 0.001 when compared with normal group and ∗∗,∗∗∗: P < 0.01, P < 0.001 when compared with 0.4% DNCB group. (c) The number of scratching behavior within 30 min was counted 1 h after each sample treatment, 3 times a week. Weekly data are expressed as mean ± SD of each scratching numbers of three days in a week. 0.01, ###
P <0.001, significantly different from the normal group. *P < 0.05, **P <0.01,***P < 0.001, significantly different from the 0.4% DNCB group.