Levels of serum (a) IgE, (b) IgG1 (c) IgG2a and ratio of serum (d) IgG1/IgG2a. (a) On the 0, 2, and 4 weeks treatment, serum was collected after mice were sacrificed. Serum level of IgE was measured using ELISA kit. (b), (c) On day 28 of sample treatment, serum was collected after mice were sacrificed. Serum levels of (b) IgG1 and (c) IgG2a were measured using ELISA kit, (d) The IgG1/IgG2a ratio in serum was calculated as IgG1/IgG2a for each mice. Data are expressed as means ± SD of five mice per group. #
P < 0.05, ##
P < 0.01, ###
P < 0.001, significantly different from the normal group. *P < 0.05, **P < 0.01,***P < 0.001, significantly different from the AD group.