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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: J Pharmacol Sci. 2010 Mar 20;112(4):467–472. doi: 10.1254/jphs.09346sc

Fig. 1.

Fig. 1

Determination of transcriptional-initiation site of the rat RhoA gene using 5′-RACE. The published RhoA cDNA sequence (GenBank accession number BC061732) was used as the reference sequence. A) Amplification of 5′-end of the rat RhoA gene by PCR. The primer sets used for the amplification were as follows: lane 1: GeneRacer™5′ and GSP1 primers, lane 2: GeneRacer™5′ and GSP2 primers, and lane 3: GeneRacer™5′ nested primer and GSP1 primer (see the text). B) Sequence of the 5′-RACE PCR products. The newly isolated 5′-end was identified at 66-bp upstream of the end of the published rat RhoA cDNA.