Dish/Plate Size	Volume of PolyHEMA (to be applied twice)	Culture Media Final Volume
6-well plate	3 ml	5-7 ml
12-well plate	1.5 ml	2 ml
24-well plate	500 μl	1.5 ml
48-well plate	250 μl	500 μl
96-well plate	50 μl	200 μl
P100 culture dish	4-5 ml	20 ml
P60 culture dish	2-3 ml	5-7 ml
F25 flask	2-3 ml	7 ml
F75 flask	4-5 ml	20-30 ml
F175 flask	15 ml	30-50 ml