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. 2012 Nov 1;7(11):e48431. doi: 10.1371/journal.pone.0048431

Figure 2. Transient neonatal olfactory deprivation affects the early differentiation phase of CR+ PGCs in the OB. A:

Figure 2

Experimental procedure. Mice undergoing 2-week hemilateral naris occlusion (P5–P19) were injected with BrdU just before naris reopening by removal of the nasal plug, and the number of BrdU+CR+ PGCs in the glomerular layer was quantified 2 weeks (2 w) and 3 weeks (3 w) later, at P33 and P40. B–C: Effect of transient neonatal olfactory deprivation on newly generated CR+ PGCs. Coronal sections of the glomerular layer in the OB 2 weeks (n = 3) and 3 weeks (n = 3) after BrdU injection were prepared and immunostained for BrdU (red) and CR (green) (B). Arrowheads in B indicate BrdU+CR+ cells. The number of BrdU+CR+ PGCs was significantly smaller in the occl. & reopening group (green bars) compared with the control, non-occluded group (blue bars) 3 weeks (P40), but not 2 weeks (P33), after BrdU injection. *P<0.05. Scale bar: 50 µm.