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. 2012 Nov 1;8(11):e1002988. doi: 10.1371/journal.ppat.1002988

Figure 5. The LecE and the Nem1-Spo7 phosphatase complex lethal effect on yeast growth are suppressed by the same yeast genetic backgrounds.

Figure 5

(A) Over expression of Dgk1 suppresses the lethal effect caused by the over expression of the Nem1-Spo7 phosphatase complex in yeast, similarly to LecE. LecE (upper two rows) or Nem1 and Spo7 (lower two rows) were over expressed in wild-type S. cerevisiae BY4741 (W.T.) together with Dgk1 or with pGREG506 (vector). (B) Deletion of pah1 suppressed the lethal effect caused by the over expression of the Nem1-Spo7 phosphatase complex in yeast, similarly to LecE. LecE (upper two rows) or Nem1 and Spo7 (lower two rows) were over expressed in wild-type S. cerevisiae or in the pah1 deletion mutant RV-L8-59 (pah1Δ). (C) Over-expression of the Pho85-Pho80 kinase-cyclin complex suppresses the lethal effect caused by the over expression of the Nem1-Spo7 phosphatase complex in yeast, similarly to LecE. LecE (upper two rows) or Nem1 and Spo7 (lower two rows) were over-expressed in wild-type S. cerevisiae W303 (W303) together with Pho85 and Ph080 or with the relevant vectors. The experiment was preformed at 30°C.