Figure 2.

Mapping the core promoter region of human Sell gene. (A) 5′ serial deletion mutants shown on the left side were transiently transfected into Jurkat (stripped bars), EL4 (open bars), or HeLa cells (solid bars) and Luciferase activity shown on the right side was analyzed 30 hours after transfection. (B) Jurkat cells were co-transfected with core promoter construct, hSell288, with plasmids expressing Sp1, Mzf1, Klf2, Irf1, Ets1.

Notes: Luciferase activity was analyzed 30 hours after the co-transfection. Luciferase activity was expressed as percentage of that of pGL3-Promoter in 2A and as fold changes relative to that of pGL3 vector transfected Jurkat cells in 2B. Data shown are mean ± SD of three independent transfections in one experiment. Each experiment was repeated at least three times.