Figure 3.

Constitutive active FOXO1 up-regulates endogenous human Sell expression. Jurkat cells were transiently transfected with pcDNA3 (white bar), pcDNA3-FOXO1 (striped bar), or pcDNA3-FOXO1-3A (solid bar) for 30 hours. Of the two sets of transfected cells, one set was lysed with SDS buffer and equal amount of lysate from each treatment was analyzed for expression of FOXO1 by Western blot, where β-actin was used as loading control (A); the other set was used to analyze the expression of human Sell by real time PCR, which was normalized to that of GAPDH (B).

Notes: Data were presented as mean ± SD of at least three independent experiments in triplicate on each transfection. Data were graphed as fold increase relative to that of pcDNA3 transfected Jurkat cells, which was set as 1.