Figure 5.

Locating the FOXO1 motif.

Notes: Jurkat cells were transiently co-transfected with either pcDNA3 (open bars) or FOXO1-3A (solid bars) and one of the 5′ serial deletion mutants as labeled. Luciferase activity normalized to that of Renilla activity was analyzed 30 hours after the co-transfection. Data were presented as mean ± SD of at least three independent experiments in triplicate on each transfection. Data were graphed as fold increase relative to that of pcDNA3 co-transfected Jurkat cells, which was set as 1.