Figure 6.

FOXO1 transactivates human Sell promoter through binding to the motif. (A) Alignment of the IRE with the FOXO1 motifs in the Sell promoter of both human and chimpanzee. (B) Jurkat cells were transiently co-transfected with the combination of plasmids, pcDNA3 and hSell108, FOXO1-3A and hSell108, or FOXO1-3A and hSellFmut, as labeled on the bottom.

Notes: Luciferase activity was analyzed 30 hours after transfection. Luciferase activity expressed as fold increase relative to that of pcDNA3 transfected cells. Data shown are mean ± SD of three independent transfections in one experiment. Each experiment was repeated at least three times in triplicates.