Figure 7.

FOXO1 binds to FOXO1 motif in vitro.

Notes: DNA probe containing IRE from APOC3 gene was labeled with γ-³²P-ATP (lane 1), which was then incubated with nuclear extract from HeLa cells over-expressing FOXO1-3A (lane 2). The protein-DNA complex was then competed with either 10× and 100× cold APOC3 probe (lane 3 and 4, labeled as APOC3), or with 10× and 100× cold probe containing FOXO1 motif from human Sell (lane 5 and 6, labeled as FOXO1), or with 10× and 100× cold probe containing mutated FOXO1 motif from human Sell (lane 7 and 8, labeled as FOXO1m). Free probe was indicated with arrowhead and specific DNA-protein complex was indicated with arrow on the side.