Primers for constructs |
For.1088 |
5′-ATAGCTCGAGTAACCTCTTTGAGACTCT-3′ |
For.488 |
5′-ATAGCTCGAGGAAGGAGGAAGAGGA-3′ |
For.288 |
5′-ATAGCTCGAGCTGATCAGCAGTTCATT-3′ |
For.188 |
5′-ATAGCTCGAGAAAAGGGGAGGAGGAGGA-3′ |
For.108 |
5′-ATAGCTCGAGTCTACCTGCAGCACAGCA-3′ |
Rev. |
5′-CTACAGATCTGGCTTTGCTTGGTCCT-3′ |
FOXO1m |
5′-GGGTCTCAGGTCCTTGCCTTCGTTGAGTGTGCTGTGCTGCAG-3′ |
Primers for real time PCR |
For.Sell |
5′-GGCAGCCCTCTGTTACACA-3′ |
Rev.Sell |
5′-ACATCACAGTTGCAGGTGTA-3′ |
For.GAPDH |
5′-CATGAGAAGTATGACAACAGCCT-3′ |
Rev.GAPDH |
5′-AGTCCTTCCACGATACCAAAGT-3′ |
Probes (shown only sense strand) |
APOC3 |
5′-CCTTTACTCCAAACACCCCCCA-3′ |
FOXO1 |
5′-GCACACTCCCTTTGGGCAAGGA-3′ |
FOXO1m |
5′-GCACACTCAACGAAGGCAAGGA-3′ |