Table 1. Transformation frequencies of S. aureus strains.
Recipient strain | ||||||
N315h | N315ΔcomEh | N315ΔcomGh | N315 | N315ex w/oφ | N315ex w/oφ | |
pRIT-sigH | pRIT5H | |||||
Donor | ||||||
4.0×10−9±3×10−9 | ||||||
Plasmid DNA (10 µg pT181) | (n = 11) | |||||
ND | ND | ND | ND | |||
(n = 1) | (n = 3) | (n = 3) | (n = 3) | |||
9.0×10−7±6.6×10−9 | 2×10−10 | |||||
(n = 12) | (n = 1) | |||||
COL/pT181 | ND | ND | ND | |||
(n = 5) | (n = 4) | (n = 9) | ||||
Plasmid DNA (10 µg pHY300) | 1.5×10−6±6.3×10−7 | ND | ||||
(n = 3) | (n = 2) |
Frequencies = number of transformants/cfu of recipient strain; mean ± SD.
ND: none detected.