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. 1980 Apr;77(4):2255–2259. doi: 10.1073/pnas.77.4.2255

Unreliability of tritiated cholesterol: studies with [1,2-3H]-cholesterol and [24,25-3H]cholesterol in humans.

N O Davidson, E H Ahrens Jr, H L Bradlow, D J McNamara, T S Parker, P Samuel
PMCID: PMC348692  PMID: 6929548

Abstract

Over the past 18 months different lots of [1,2-3H]cholesterol and [24,25-3H]cholesterol were found to be radiochemically acceptable by conventional chemical and other in vitro tests, yet, when co-administered with [4-14C]cholesterol to human subjects, an abrupt fall in the 3H/14C specific activity ratio in plasma cholesterol was discovered in every case. We have concluded that all batches of [3H]cholesterol should be regarded as radiochemically unreliable unless they are shown to behave identically in all respects to [4-14C]cholesterol in an appropriate in vivo assay system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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