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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Pulm Pharmacol Ther. 2012 Jun 16;25(6):427–431. doi: 10.1016/j.pupt.2012.06.003

Figure 5.

Figure 5

Western Blot probing for YFP (using antibody against GFP) in HBE1 cells transfected with YFP fused to the N-terminus of MARCKS. A) the entire blot; B) the area of the blot within the dotted line in “A”; this region was exposed longer to bring out blot details; C) actin used for normalization. Lanes 1,2: MARCKS constructs; Lanes 3,4: empty vector. Treatment of cells for 3 min with 100 μM ATP (B; lane 2) shows apparent induction of MARCKS cleavage products of molecular weight 27 - 32 kDa (indicated by arrows).