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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Hepatology. 2012 Jul 26;56(5):1946–1957. doi: 10.1002/hep.25873

Fig. 5. Circulating miRNAs localize to the exosome-rich or protein-rich compartments in a liver injury-specific manner.

Fig. 5

Exosome-rich or protein-rich fractions were isolated with ExoQuick Exosome Precipitation solution (SBI, System Biosciences) from serum or plasma as described in methods and lysed with QIAzole (Qiagen). Total RNA was isolated with miRNeasy kit (Qiagen) and used for miRNA detection. The expression of miR-122 and miR-155 was quantified in exosome-rich or protein-rich fractions isolated from serum (A left & right; ALD model) and plasma (C left & right; APAP model and E left & right; CpG model). B&D&F Expression of miR-122 and miR-155 was quantified in the liver after alcohol (B), APAP (D) and CpG+LPS (F) treatments (n=4–8). Spiked C. elegans miR-39 (serum or plasma) and SnoRNA202 (liver) was used as internal control. The fold change over pair-fed (5A,B) or saline (5C–F) treated mice is shown. Data represent mean ± SEM. * p<0.05 compared to saline treated mice. Non-parametric Mann-Whitney test was employed for statistical analysis.