Fig. (2). Effects of mutations at splice sites in the BTK gene on splicing events.

Splicing events in BTK transcripts were examined using RT-PCR products, luciferase activity, and HaloTag fusion proteins. We examined the effects of a G>T change at position –1 of the 3’-splicing acceptor site in intron 16 of the BTK gene. The experiment was carried out in triplicate and RT-PCR products were quantified as weighted ratios obtained from a MCE-202 MultiNA Microchip Electrophoresis System. Schematic representations of the RT-PCR products are shown on the right in the figure.