Fig. (3). Effects of mutations at splice sites in the WAS gene on splicing events.

Splicing events in WAS transcripts were assessed using RT-PCR products, luciferase activity, and HaloTag fusion proteins. We used exon/intron cassette-trapping plasmids with or without mutations (G>A, G>T, G>C, or wild-type) at position +5 of the 5’-splicing donor site in intron 6 of the WAS gene. The experiment was carried out in triplicate and RT-PCR products were quantified as weighted ratios obtained from a MCE-202 MultiNA Microchip Electrophoresis System. Schematic representations of the RT-PCR products are shown on the right in the figure.