Fig. (6). Effects of splice-site mutations in CYBB exon/intron cassettes containing a single intron.

Splicing events identified based on RT-PCR products, luciferase activity, and HaloTag fusion proteins are shown. We used exon/intron cassette-trapping plasmids with or without mutations (G>C, G>T, G>A, or wild-type) at position +5 of the 5’-splicing donor site in intron 5 of the CYBB gene. The experiment was carried out in triplicate and levels of RT-PCR products were quantified as the weighted ratios obtained using a MCE-202 MultiNA Microchip Electrophoresis System. Schematic representations of the RT-PCR products are shown on the right in the figure.