Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Composition of dissection and recording solutions
| mM | Solution 1 | Solution 2 | Solution 3 | HBS |
|---|---|---|---|---|
| NaCl | 130 | — | 130 | 135 |
| Potassiu gluconate | — | 130 | — | — |
| KCl | 2.5 | 15 | 2.5 | 4 |
| NaH2PO4 | 1.3 | — | 1.3 | — |
| EGTA | — | 0.5 | — | — |
| Hepes | — | 18 | — | 10 |
| Glucose | 25 | 25 | 25 | 25 |
| Sucrose | 230 | — | — | — |
| NaHCO3 | 26 | — | 26 | 2 |
| Ascorbic acid | 0.395 | — | 0.395 | — |
| CaCl2 | 0.8 | — | 2 | 2 |
| MgCl2 | 8 | — | 1 | 2 |
| Kynurenic acid | 1 | 1 | — | — |
| Sodium pyruvate | 2 | 2 | 2 | — |
| KOH | — | 8.7 | — | — |
Solutions 1 to 3 were bubbled with 95% O2–5% CO2. pH was adjusted to 7.4 in all the solutions.
