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. 2012 Jul 23;590(Pt 19):4717–4733. doi: 10.1113/jphysiol.2012.234229

Figure 5. CP 55,940-mediated inhibition of high-threshold primary afferent EPSCs is absent in CB1−/− and sns-CB1−/− mice.

Figure 5

A, lack of inhibition by CP 55,940 (3 μm) of high-threshold EPSCs in CB1−/− mice. Left, time course. Right, averages traces of 10 consecutive EPSCs before application of CP 55,940 (black, control) and after (CP, red). B, same as A but in nociceptor-specific sns-CB1−/− mice. C, CP 55,940-induced depression of high-threshold EPSCs in wild-type mice was not reversed by AM 251 (50 μm). Left, time course. Right, averages traces of 10 consecutive EPSCs before application of CP 55,940 (black, control), after application of CP 55,940 (CP, red) and in the additional presence of AM 251 (CP AM, grey). D, statistics: normalised high-threshold EPSC amplitudes relative to pre-treatment values. Wild-type (wt, CB1fl/fl) are same as those shown in Fig. 4A. AM 251 was applied after inhibition by CP 55,940 had been established. ***P≤ 0.001; *P≤ 0.05 significantly different from wild-type, ANOVA followed by Dunnett's post hoc test.