Figure 4. The currents evoked by pH 7.0 are amiloride sensitive.

A, voltage clamp recording from an NTS neuron in acute slice. Focal application of pH 7.0 induced a desensitizing inward current that was substantially reduced by co-application of pH 7.0 and 100 μm amiloride. B, bar chart summarizing the results obtained in 3 different neurons. C, focal application of pH 7.0 in acute brain slices elicits a transient depolarization that is reversibly inhibited by 100 μm amiloride. D, bar chart depicting the net depolarization during focal application of pH 7.0 alone and in the presence of 100 μm amiloride (*P < 0.05; one-tailed Student's t test).