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In Situ Hybridization of the PNPT1 Ortholog in Zebrafish
(A) Maternal expression of pnpt1 in 2-cell-stage fertilized zebrafish eggs.
(B–C) At 50% and 80% epiboly, pnpt1 mRNA is broadly expressed in the whole embryo.
(D) 24 hr postfertilization (hpf) expression is highest in the head area and disappears toward the tail.
(E–F) 48 hpf and 120 hpf expression becomes restricted to the tectum, the gill arches, and the developing ear (indicated by arrows).
